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Atlas of Living Cell Cultures by Prof. Dr. Toni Lindl, Dr. Rosemarie Steubing(auth.) PDF

By Prof. Dr. Toni Lindl, Dr. Rosemarie Steubing(auth.)

ISBN-10: 3527328874

ISBN-13: 9783527328871

ISBN-10: 3527669922

ISBN-13: 9783527669929

The 1st atlas in lots of years giving researchers a great visible reference of the prestige in their mobilephone strains. Given the expanding value of good outlined mobile versions particularly in biomedical examine it is a sorely wanted source for everybody appearing cellphone culture.Content:
Chapter 1 creation (pages 1–4):
Chapter 2 simple cellphone tradition strategies (pages 5–9):
Chapter three checklist of telephone traces and Human fundamental Cells (in Alphabetical Order) (pages 11–14):
Chapter four cellphone strains and Human fundamental Cells (pages 15–492):

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Extra info for Atlas of Living Cell Cultures

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Et al. (1973) In vitro cultivation of human tumors: establishment of cell lines derived from a series of solid tumors. J. Natl. , 51, 1417–1423. CHT04AAC 03/20/2013 22:0:52 Page 24 A-375, 100Â Leica. A-375, 100Â Leica. A-375, 400Â Leica. Atlas of Living Cell Cultures, First Edition. Toni Lindl and Rosemarie Steubing. # 2013 Wiley-VCH Verlag GmbH & Co. KGaA. Published 2013 by Wiley-VCH Verlag GmbH & Co. KGaA. J. 02% EDTA solution. 02% EDTA solution and let the culture sit at 37  C until the cells detach.

Et al. (1977) Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. , 58, 209–214. CHT04AAC 03/20/2013 22:0:56 Page 32 A-549, 100Â Leica. A-549, 100 Â Leica. A-549, 400Â Leica. Atlas of Living Cell Cultures, First Edition. Toni Lindl and Rosemarie Steubing. # 2013 Wiley-VCH Verlag GmbH & Co. KGaA. Published 2013 by Wiley-VCH Verlag GmbH & Co. KGaA. CHT04AAC 03/20/2013 22:0:56 Page 33 Human Cell Lines A-549 A Origin and General Characteristics Organism: Ethnicity: Age: Gender: Tissue: Morphology: Cell type: Growth properties: Description: Homo sapiens (human) Caucasian 58 years Male Lung Epithelial Carcinoma Monolayer The cells are positive for keratin by immunoperoxidase staining Culture Conditions and Handling Culture medium: Subculture routine: Split ratio: Fluid renewal: Biosafety level: DMEM:Ham’s F12 medium supplemented with 2 mM L-glutamine and 10% fetal bovine serum.

02% EDTA and incubate at 37  C until cells detach. Y. and Ozello, L. (1958) Cultivation of human breast carcinomas. J. Natl. , 21 (6), 1131–1147. CHT04AAC 03/20/2013 22:1:2 Page 46 BT-474, 100Â Leica. BT-474, 100Â Leica. BT-474, 400Â Leica. Atlas of Living Cell Cultures, First Edition. Toni Lindl and Rosemarie Steubing. # 2013 Wiley-VCH Verlag GmbH & Co. KGaA. Published 2013 by Wiley-VCH Verlag GmbH & Co. KGaA. CHT04AAC 03/20/2013 22:1:2 Page 47 Human Cell Lines BT-474 B Origin and General Characteristics Organism: Ethnicity: Gender: Age: Tissue: Cell type: Morphology: Growth properties: Description: Homo sapiens (human) Caucasian Female 60 years Breast Mammary gland Epithelial Monolayer; the cells form compact, multilayered colonies; growing slowly, and rarely becoming confluent The BT-474 line was isolated by E.

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